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Veröffentlichungen von Ian Levenfus:

 

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Levenfus I1, Ullmann U2, Battegay E1, Schuurmans M3

1 University Hospital Zurich, Division of Internal Medicine, Zurich, Switzerland; University of Zurich, Faculty of Medicine, Zurich, Switzerland.

2 University of Leipzig Medical Center, Psychotherapy and Psychosomatics, Department of Pediatric Psychiatry, Leipzig, Germany; Technische Univeristat Dresden, Department of Medicine, Dresden, Germany; South Ural State University, Department of Medical Biology, Chelyabinsk, Russia.

3 University of Zurich, Faculty of Medicine, Zurich, Switzerland; University Hospital Zurich, Division of Pulmonology, Zurich, Switzerland.

Abstract

Clinical prediction scores support the assessment of patients in the emergency setting to determine the need for further diagnostic and therapeutic steps. During the current COVID-19 pandemic, physicians in emergency rooms (ER) of many hospitals have a considerably higher patient load and need to decide within a short time frame whom to hospitalize. Based on our clinical experiences in dealing with COVID-19 patients at the University Hospital in Zurich, we created a triage score with the acronym "AIFELL" consisting of clinical, radiological and laboratory findings. The score was then evaluated in a retrospective analysis of 122 consecutive patients with suspected COVID-19 from March until mid-April 2020. Descriptive statistics, Student's t-test, ANOVA and Scheffe's post-hoc analysis confirmed the diagnostic power of the score. The results suggest that the AIFELL score has potential as a triage tool in the ER setting intended to select probable COVID-19 cases for hospitalization in spontaneously presenting or referred patients with acute respiratory symptoms.

Keywords: Cell culture, Signal transduction, Differentiation, Self-renewal

Published: 2020-09-01

Levenfus I, Ullmann E, Battegay E, Schuurmans MM. Triage tool for suspected COVID-19 patients in the emergency room: AIFELL score [published online ahead of print, 2020 Aug 20]. Braz J Infect Dis. 2020;S1413-8670(20)30102-1. doi:10.1016/j.bjid.2020.07.003

PMID: 32828735

 


Masjkur J1Levenfus I1, Lange S1, Arps-Forker C1, Poser S1, Qin N1, Vukicevic V1, Chavakis T1, Eisenhofer G1, Bornstein SR1, Ehrhart-Bornstein M1, Androutsellis-Theotokis A1.

1 Department of Internal Medicine III and Institute of Clinical Chemistry and Laboratory Medicine, University Clinic Carl-Gustav Carus, University of Dresden, Dresden, Germany; Center for Regenerative Therapies Dresden, Dresden, Germany.

Abstract

We present a method to efficiently culture primary chromaffin progenitors from the adult bovine adrenal medulla in a defined, serum-free monolayer system. Tissue is dissociated and plated for expansion under support by the mitogen basic fibroblast growth factor (bFGF). The cultures, although not homogenous, contain a subpopulation of cells expressing the neural stem cell marker Hes3 that also propagate. In addition, Hes3 is also expressed in the adult adrenal medulla from where the tissue is taken. Differentiation is induced by bFGF withdrawal and switching to Neurobasal medium containing B27. Following differentiation, Hes3 expression is lost, and cells acquire morphologies and biomarker expression patterns of chromaffin cells and dopaminergic neurons. We tested the effect of different treatments that we previously showed regulate Hes3 expression and cell number in cultures of fetal and adult rodent neural stem cells. Treatment of the cultures with a combination of Delta4, Angiopoietin2, and a Janus kinase inhibitor increases cell number during the expansion phase without significantly affecting catecholamine content levels. Treatment with cholera toxin does not significantly affect cell number but reduces the ratio of epinephrine to norepinephrine content and increases the dopamine content relative to total catecholamines. These data suggest that this defined culture system can be used for target identification in drug discovery programs and that the transcription factor Hes3 may serve as a new biomarker of putative adrenomedullary chromaffin progenitor cells.

Keywords: Cell culture, Signal transduction, Differentiation, Self-renewal

Published: 2014-05-22

Masjkur J, Levenfus I, Lange S et al. 2014. A defined, controlled culture system for primary bovine chromaffin progenitors reveals novel biomarkers and modulators. Stem Cells Transl. Med. 3: 801–8.

PMID: 24855275


Levenfus, I.

Stem Cell Biology, Medical Clinic III, Medical Faculty Carl Gustav Carus

Abstract

Counting cells is a crucial procedure in evaluating the success of a treatment. A fast yet exact way of doing this for DAPI-stained cells with the ImageJ-derivate ‘Fiji’ is described in this paper, written as a step-by-step tutorial with screenshots.

Keywords: cell counting, quantity, DAPI, Fiji, ImageJ, tutorial

Published: 2011-03-11, 17:44

Last revision: 2011-03-11, 17:44

Levenfus, I.: An efficient method for counting DAPI-stained cells using Fiji. IL-PubServ: Dresden 2011.

Also offered by GRIN: Levenfus, I.: An efficient method for counting DAPI-stained cells using Fiji. GRIN: München 2011.

An efficient method for counting DAPI-stained cells using Fiji (PDF-document - 903,6kB)